Metodologia 71
Tabela 2. Concentrações, clones, diluições e fabricantes dos anticorpos utilizados.
Anticorpo Clone Fabricante Diluição
Bag-1 5C5 Novocastra, Newcastle upon
Tyne, UK
1:50
Bax
Polyclonal
Dako, carpinteria, CA
1:100
Bcl-2
Bcl-
2/100/D5
Novocastra, Newcastle upon
Tyne, UK
1:50
Caspase 8 11B6
Novocastra, Newcastle upon
Tyne, UK
1:50
c-erbB-2 CB11
Novocastra, Newcastle upon
Tyne, UK
1:300
c-erbB-3 RTJ1
Novocastra, Newcastle upon
Tyne, UK
1:100
Checkpoint kinase 2 (Chk2) DCS 270.1
Novocastra, Newcastle upon
Tyne, UK
1:50
Cyclin D1 P2D11F11
Novocastra, Newcastle upon
Tyne, UK
1:50
Epidermal growth factor receptor (EGFR) EGFR.113
Novocastra, Newcastle upon
Tyne, UK
1:50
Extracellular matrix metalloproteinase inducer
(EMMPRIN)
8D6
Santa Cruz, Palo Alto, CA
1:50
Estrogen receptor (ER) 6F11
Novocastra, Newcastle upon
Tyne, UK
1:100
Human telomerase reverse transcriptase
(hTERT)
44F12
Novocastra, Newcastle upon
Tyne, UK
1:100
Ki67 MM1
Novocastra, Newcastle upon
Tyne, UK
1:150
Matrix metalloproteinase 1 (MMP1) 3B6
Novocastra, Newcastle upon
Tyne, UK
1:50
Matrix metalloproteinase 2 ( MMP2)
8B4
Santa Cruz, Palo Alto, CA
1:50
Osteopontin (OPN)
AKm2A1
Santa Cruz, Palo Alto, CA
1:100
p16 6H12
Novocastra, Newcastle upon
Tyne, UK
1:50
p21 4D10
Novocastra, Newcastle upon
Tyne, UK
1:50
p27 1B4
Novocastra, Newcastle upon
Tyne, UK
1:50
p53 DO-7
Novocastra, Newcastle upon
Tyne, UK
1:100
p63
4A4
Santa Cruz, Palo Alto, CA
1:100
Plasminogen activator inhibitor (PAI) TJA6
Novocastra, Newcastle upon
Tyne, UK
1:50
Progesterone receptor 1A6
Novocastra, Newcastle upon
Tyne, UK
1:150
Survivin FL-142
Santa Cruz, Palo Alto, CA
1:100
Tissue inhibitor of matrix metalloproteinase 1
(TIMP1)
6F6a
Novocastra, Newcastle upon
Tyne, UK
1:100
Tissue inhibitor of matrix metalloproteinase 2
(TIMP2)
3A4
Novocastra, Newcastle upon
Tyne, UK
1:100
Vascular endothelial growth factor (VEGF) A-20
Santa Cruz, Palo Alto, CA
1:200