134 pb untranslated 5´sequence (5´UTR)
AATAACACAACCACACAAAGGCTTCAGAGAGTTGCGAAAGCAGATTAGCTCCTCTCTTTGTTTGGCTTTGGGCCTTTTGGGTTCTCTTCTCG
TTTTAATCATCCACAGTTCTCTCCCCTTCCTGCTAACAAA
1 ATGTTCATCGACACGTTTAAGGTTGAGAGTCCTAACGTTAAGTACACAGAGGATGAGATT
1 M F I D T F K V E S P N V K Y T E D E I
61 CACTCGGTGTACAACTATGAGACCACTGAGCTTGTTCATGAGAACAGGAATGGTACCTAT
21 H S V Y N Y E T T E L V H E N R N G T Y
121 CAGTGGACTGTCAAACCCAAAACTGTCCAATACGAATTCAAGACCAGCATTCATGTCCCC
41 Q W T V K P K T V Q Y E F K T S I H V P
181 AAACTCGGGGTTATGCTCGTGGGGTGGGGAGGAAACAATGGATCGTCTCTCACCGGCGGT
61 K L G V M L V G W G G N N G S S L T G G
241 ATCATAGCCAACCGAGAGGGAATCTCCTGGGCGACCAAGGACAAGGTGCAGCAAGCCAAC
81 I I A N R E G I S W A T K D K V Q Q A N
301 TACTTTGGCTCGCTAACCCAGGCTTCAACTATCCGAGTTGGGTCTTTCAATGGAGAGGAG
101 Y F G S L T Q A S T I R V G S F N G E E
361 ATTTATGCTCCATTCAAAAGCCTGCTCCCCATGGTGAGCCCCGATGACGTTGTTATTGGA
121 I Y A P F K S L L P M V S P D D V V I G
421 GGATGGGACATAAGTGACATGAACTTGGCTGACGCCATGGCCAGGGCAAAAGTCTTCGAC
141 G W D I S D M N L A D A M A R A K V F D
481 ATTGATCTGCAGAAACAACTCAGGCCCTACATGGAATCCATGATCCCACTACCCGGAATA
161 I D L Q K Q L R P Y M E S M I P L P G I
541 TATGACCCTGATTTCATTGCTGCCAACCAAGGGTCACGTGCCAACAATGTGATCACAGGC
181 Y D P D F I A A N Q G S R A N N V I T G
601 TCCAAGAAAGAACAACTTCAGCAAGTCATCAAGGACATCAGGGAGTTTAAGGAAAAAAAC
201 S K K E Q L Q Q V I K D I R E F K E K N
661 AAGGTAGACAAGGTGGTTGTGCTGTGGACTGCCAACACAGAGAGGTACAGCAATGTCGTC
221 K V D K V V V L W T A N T E R Y S N V V
721 GTGGGGCTAAATGACACCATGGAGAACCTCATGGCTGCACTGGAGAGGAATGAATCAGAG
241 V G L N D T M E N L M A A L E R N E S E
781 ATATCTCCATCAACTTTGTATGCCTTGGCCTGTGTGTTGGAAAATGTTCCTTTTGTGAAC
261 I S P S T L Y A L A C V L E N V P F V N
841 GGAAGCCCCCAGAACACCTTTGTTCCAGGGTTGATTGATCTGGCTATCAAGAGGAACAGT
281 G S P Q N T F V P G L I D L A I K R N S
901 TTGATTGGTGGGGATGACTTTAAGAGTGGTCAGACCAAAATGAAGTCCGTCCTGGTGGAT
301 L I G G D D F K S G Q T K M K S V L V D
961 TTCCTTGTTGGTGCTGGGATCAAGCCAACGTCGATAGTGAGCTACAACCATCTGGGCAAT
321 F L V G A G I K P T S I V S Y N H L G N
1021 AATGACGGCATGAATCTGTCAGCGCCGCAAACTTTCCGTTCCAAGGAGATCTCCAAGAGC
341 N D G M N L S A P Q T F R S K E I S K S
1081 AATGTTGTAGACGACATGGTCTCCAGCAATGGAATACTCTATGAACCTGGTGAACATCCT
361 N V V D D M V S S N G I L Y E P G E H P
1141 GACCACGTTGTGGTCATTAAGTATGTGCCATACGTGGGGGATAGCAAGAGGGCTATGGAC
381 D H V V V I K Y V P Y V G D S K R A M D
1201 GAGTACACCTCGGAGATATTCATGGGTGGAAAGAGCACTATAGTGCTACACAATACGTGT
401 E Y T S E I F M G G K S T I V L H N T C
1261 GAAGACTCCCTGCTGGCTGCACCCATCATCCTGGACTTGGTTCTTCTCGCTGAGCTCAGT
421 E D S L L A A P I I L D L V L L A E L S
1321 ACCAGGATCCAGCTGAAAGCCGAGGGAGAGGGAAAGTTTCATTCTTTCCACCCGGTGGCT
441 T R I Q L K A E G E G K F H S F H P V A
1381 ACCATCCTCAGTTACCTCACAAAGGCTCCTCTGGTTCCCCCAGGGACGCCGGTAGTGAAT
461 T I L S Y L T K A P L V P P G T P V V N
1441 GCACTTTCAAAGCAGCGTGCAATGCTGGAGAACATATTGAGAGCTTGTATTGGATTGGCT
481 A L S K Q R A M L E N I L R A C I G L A
1501 CCAGAGAATAACATGATTTTGGAATACAAGTGAAGTATTAAGAAGAATAACAAGGATGAT
501 P E N N M I L E Y K *
1561 GGTGGGGAGCAGACCAGTTCATAAGCTGTAGAAACAATGAATGTTCCCTTTTCTCTTTCC
AATCCAGTGTTTATTGATGTCTGTCTGATATTTGCCATTACTAGGCATATTAGTTCTCTATGTTCTGGCTTTTAGCGTTGTGTAGGCTTTTA
GCCCCTCTGCTCTGGCATTTTCAATGACCGAGCTACTCTTTTCATGTGGAAATAGTTCATAAATATAAAACTGTCTATAAATTCTGTTCAAA
AAAAAAAAAAAAAAA
268 pb untranslated 3´sequence (3´UTR)
Figura 6. Seqüência completa do cDNA do gene PeMIPS e da proteína deduzida in
sílico. A seqüência 5´ UTR e 3´ UTR possuem 134 e 268 pb, respectivamente. O realce em
vermelho indica os codons de iniciação e terminação. O realce em amarelo os sinais de
poliadenilação. E as letras em azul a cauda poli (A).
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