Lipid raft proteome reveals ATP synthase complex in the cell surface. Pro-
teomics 4:3536–3548.
2. Barros, T. F., and R. Puccia. 2001. Cloning and characterization of a LON
gene homologue from the human pathogen Paracoccidioides brasiliensis.
Yeast 18:981–988.
3. Bialek, R., A. Ibricevic, A. Fothergill, and D. Begerow. 2000. Small subunit
ribosomal DNA sequence shows Paracoccidioides brasiliensis closely related
to Blastomyces dermatitidis. J. Clin. Microbiol. 38:3190–3193.
4. Bota, D. A., J. K. Ngo, and K. J. Davies. 2005. Downregulation of the human
Lon protease impairs mitochondrial structure and function and causes cell
death. Free Radic. Biol. Med. 38:665–677.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of
microgram quantities of protein utilizing the principle of protein-dye bind-
ing. Anal. Biochem. 72:248–254.
6. Carbonell, L. M., and J. Rodriguez. 1968. Mycelial phase of Paracoccidioides
brasiliensis and Blastomyces dermatitidis: an electron microscope study. J.
Bacteriol. 96:533–543.
7. Carbonell, L. M. 1969. Ultrastructure of dimorphic transformation in Para-
coccidioides brasiliensis. J. Bacteriol. 100:1076–1082.
8. Carvalho, K., L. Ganiko, W. L. Batista, F. V. Morais, E. Marques, G. H.
Goldman, M. F. Franco, and R. Puccia. 2005. Virulence of Paracoccidioides
brasiliensis and gp43 expression in isolates bearing known PbGP43 geno-
types. Microbes Infect. 7:55–65.
9. Casanova, M., and W. L. Chaffin. 1991. Cell wall glycoproteins of Candida
albicans as released by different methods. J. Gen. Microbiol. 137:1045–1051.
10. Chaffin, W. L., J. L. Lopez-Ribot, M. Casanova, D. Gozalbo, and J. P.
Martinez. 1998. Cell wall and secreted proteins of Candida albicans: identi-
fication, function, and expression. Microbiol. Mol. Biol. Rev. 62:130–180.
11. Cisalpino, P. S., R. Puccia, L. M. Yamauchi, M. I. Cano, J. F. da Silveira,
and L. R. Travassos. 1996. Cloning, characterization, and epitope expression
of the major diagnostic antigen of Paracoccidioides brasiliensis. J. Biol. Chem.
271:4553–4560.
12. Fan, C. Y., S. Lee, and D. M. Cyr. 2003. Mechanisms for regulation of Hsp70
function by Hsp40. Cell Stress Chaperones 8:309–316.
13. Feitosa, L. S., P. S. Cisalpino, M. R. dos Santos, R. A. Mortara, T. F.
Barros, F. V. Morais, R. Puccia, J. F. da Silveira, and Z. P. de Camargo.
2003. Chromosomal polymorphism, syntenic relationships, and ploidy in
the pathogenic fungus Paracoccidioides brasiliensis. Fungal Genet. Biol.
39:60–69.
14. Felipe, M. S., R. V. Andrade, F. B. Arraes, A. M. Nicola, A. Q. Maranhao,
F. A. Torres, I. Silva-Pereira, M. J. Pocas-Fonseca, E. G. Campos, L. M.
Moraes, P. A. Andrade, A. H. Tavares, S. S. Silva, C. M. Kyaw, D. P. Souza,
P. Network, M. Pereira, R. S. Jesuino, E. V. Andrade, J. A. Parente, G. S.
Oliveira, M. S. Barbosa, N. F. Martins, A. L. Fachin, R. S. Cardoso, G. A.
Passos, N. F. Almeida, M. E. Walter, C. M. Soares, M. J. Carvalho, and
M. M. Brigido. 2005. Transcriptional profiles of the human pathogenic
fungus Paracoccidioides brasiliensis in mycelium and yeast cells. J. Biol.
Chem. 280:24706–24714.
15. Goldman, G. H., M. E. dos Reis, R. Duarte, L. A. Souza Bernardes, A. C.
Quiapin, P. M. Vitorelli, M. Savoldi, C. P. Semighini, R. C. de Oliveira, L. R.
Nunes, L. R. Travassos, R. Puccia, W. L. Batista, L. E. Ferreira, J. C.
Moreira, A. P. Bogossian, F. Tekaia, M. P. Nobrega, F. G. Nobrega, and
M. H. Goldman. 2003. Expressed sequence tag analysis of the human patho-
gen Paracoccidioides brasiliensis yeast phase: identification of putative ho-
mologues of Candida albicans virulence and pathogenicity genes. Eukaryot.
Cell 2:34–48.
16. Holbrook, J. D., and C. J. Danpure. 2002. Molecular basis for the dual
mitochondrial and cytosolic localization of alanine:glyoxylate aminotransferase
in amphibian liver cells. J. Biol. Chem. 277:2336–2344.
17. Huang, H. C., M. Y. Sherman, O. Kandror, and A. L. Goldberg. 2001. The
molecular chaperone DnaJ is required for the degradation of a soluble
abnormal protein in Escherichia coli. J. Biol. Chem. 276:3920–3928.
18. Izacc, S. M., F. J. Gomez, R. S. Jesuino, C. A. Fonseca, M. S. Felipe, G. S.
Deepe, and C. M. Soares. 2001. Molecular cloning, characterization and
expression of the heat shock protein 60 gene from the human pathogenic
fungus Paracoccidioides brasiliensis. Med. Mycol. 39:445–455.
19. Kashino, S. S., V. L. Calich, L. M. Singer-Vermes, P. A. Abrahamsohn, and
E. Burger. 1987. Growth curves, morphology and ultrastructure of ten Para-
coccidioides brasiliensis isolates. Mycopathologia 99:119–128.
20. Kelley, W. L. 1998. The J-domain family and the recruitment of chaperone
power. Trends Biochem. Sci. 23:222–227.
21. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of
the head of bacteriophage T4. Nature 227:680–685.
22. Langer, T., C. Lu, H. Echols, J. Flanagan, M. K. Hayer, and F. U. Hartl.
1992. Successive action of DnaK, DnaJ and GroEL along the pathway of
chaperone-mediated protein folding. Nature 356:683–689.
23. Leal, C. V., B. A. Montes, A. C. Mesa, A. L. Rua, M. Corredor, A. Restrepo,
and J. G. McEwen. 2004. Agrobacterium tumefaciens-mediated transforma-
tion of Paracoccidioides brasiliensis. Med. Mycol. 42:391–395.
24. Long, K. H., F. J. Gomez, R. E. Morris, and S. L. Newman. 2003. Identifi-
cation of heat shock protein 60 as the ligand on Histoplasma capsulatum that
mediates binding to CD18 receptors on human macrophages. J. Immunol.
170:487–494.
25. Lopez-Ribot, J. L., H. M. Alloush, B. J. Masten, and W. L. Chaffin. 1996.
Evidence for presence in the cell wall of Candida albicans of a protein
related to the hsp70 family. Infect. Immun. 64:3333–3340.
26. Lu, B., T. Liu, J. A. Crosby, J. Thomas-Wohlever, I. Lee, and C. K. Suzuki.
2003. The ATP-dependent Lon protease of Mus musculus is a DNA-binding
protein that is functionally conserved between yeast and mammals. Gene
306:45–55.
27. Marques, E. R., M. E. Ferreira, R. D. Drummond, J. M. Felix, M. Menossi,
M. Savoldi, L. R. Travassos, R. Puccia, W. L. Batista, K. C. Carvalho, M. H.
Goldman, and G. H. Goldman. 2004. Identification of genes preferentially
expressed in the pathogenic yeast phase of Paracoccidioides brasiliensis, using
suppression subtraction hybridization and differential macroarray analysis.
Mol. Genet. Genomics 271:667–677.
28. McEwen, J. G., V. Bedoya, M. M. Patino, M. E. Salazar, and A. Restrepo.
1987. Experimental murine paracoccidioidomycosis induced by the inhala-
tion of conidia. J. Med. Vet. Mycol. 25:165–175.
29. Morais, F. V., T. F. Barros, M. K. Fukada, P. S. Cisalpino, and R. Puccia.
2000. Polymorphism in the gene coding for the immunodominant antigen
gp43 from the pathogenic fungus Paracoccidioides brasiliensis. J. Clin. Mi-
crobiol. 38:3960–3966.
30. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identifica-
tion of prokaryotic and eukaryotic signal peptides and prediction of their
cleavage sites. Protein Eng. 10:1–6.
31. Nimrichter, L., M. L. Rodrigues, E. G. Rodrigues, and L. R. Travassos. 2005.
The multitude of targets for the immune system and drug therapy in the
fungal cell wall. Microbes Infect. 7:789–798.
32. Nimrichter, L., M. D. Cerqueira, E. A. Leita˜o, M. Kildare, E. S. Nakayasu,
S. R. Almeida, I. C. Almeida, C. S. Alviano, E. Barreto-Bergter, and M. L.
Rodrigues. 2005. Structure, cellular distribution, antigenicity, and biological
functions of Fonsecaea pedrosoi ceramide monohexosides. Infect. Immun.
73:7860–7868.
33. Nunes, L. R., R. O. Oliveira, D. B. Leite, V. S. Silva, E. R. Marques, M. E. S.
Ferreira, D. C. D. Ribeiro, L. A. S. Bernardes, M. H. S. Goldman, R. Puccia,
L. R. Travassos, W. L. Batista, M. P. No´brega, F. G. No´brega, D. Y. Yang,
and G. H. Goldman. 2005. Transcription profiling of Paracoccidioides bra-
siliensis cells undergoing the mycelium-to-yeast transition. Eukaryot. Cell
4:2115–2128.
34. Odorizzi, G., D. J. Katzmann, M. Babst, A. Audhya, and S. D. Emr. 2003.
Bro1 is an endosome-associated protein that functions in the MVB pathway
in Saccharomyces cerevisiae. J. Cell Sci. 116:1893–1903.
35. Pellecchia, M., T. Szyperski, D. Wall, C. Georgopoulos, and K. Wuthrich.
1996. NMR structure of the J-domain and the Gly/Phe-rich region of the
Escherichia coli DnaJ chaperone. J. Mol. Biol. 260:236–250.
36. Peterson, S. W., and L. Sigler. 1998. Molecular genetic variation in Emmonsia
crescens and Emmonsia parva, etiologic agents of adiaspiromycosis, and their
phylogenetic relationship to Blastomyces dermatitidis (Ajellomyces dermatitidis)
and other systemic fungal pathogens. J. Clin. Microbiol. 36:2918–2925.
37. Rodrigues, M. L., L. R. Travassos, K. R. Miranda, A. J. Franzen, S. Rozental,
W. de Souza, C. S. Alviano, and E. Barreto-Bergter. 2000. Human antibodies
against a purified glucosylceramide from Cryptococcus neoformans inhibit
cell budding and fungal growth. Infect. Immun. 68:7049–7060.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a
laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, N.Y.
39. Savel’ev, A. S., L. A. Novikova, I. E. Kovaleva, V. N. Luzikov, W. Neupert,
and T. Langer. 1998. ATP-dependent proteolysis in mitochondria. m-AAA
protease and PIM1 protease exert overlapping substrate specificities and
cooperate with the mtHsp70 system. J. Biol. Chem. 273:20596–20602.
40. Sheffield, P., S. Garrard, and Z. Derewenda. 1999. Overcoming expression
and purification problems of RhoGDI using a family of “parallel” expression
vectors. Protein Expr. Purif. 15:34–39.
41. Silva, S. P., M. I. Borges-Walmsley, I. S. Pereira, C. M. Soares, A. R.
Walmsley, and M. S. Felipe. 1999. Differential expression of an hsp70 gene
during transition from the mycelial to the infective yeast form of the human
pathogenic fungus Paracoccidioides brasiliensis. Mol. Microbiol. 31:1039–
1050.
42. Singh, B., B. J. Soltys, Z. C. Wu, H. V. Patel, K. B. Freeman, and R. S. Gupta.
1997. Cloning and some novel characteristics of mitochondrial Hsp70 from
Chinese hamster cells. Exp. Cell Res. 234:205–216.
43. Soares, R. M., Costa e Silva-Filho, S. Rozental, J. Angluster, W. de Souza,
C. S. Alviano, and L. R. Travassos. 1998. Anionogenic groups and surface
sialoglycoconjugate structures of yeast forms of the human pathogen Para-
coccidioides brasiliensis. Microbiology 144:309–314.
44. Soltys, B. J., and R. S. Gupta. 1996. Immunoelectron microscopic localiza-
tion of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian
cells. Exp. Cell Res. 222:16–27.
45. Soltys, B. J., and R. S. Gupta. 2000. Mitochondrial proteins at unexpected
cellular locations: export of proteins from mitochondria from an evolution-
ary perspective. Int. Rev. Cytol. 194:133–196.
VOL. 5, 2006 PbMdj1 LOCALIZES TO THE MITOCHONDRIA AND CELL WALL 389